Editing sterol side chain reductase 2 gene (<i>StSSR2</i>) via CRISPR/Cas9 reduces the total steroidal glycoalkaloids in potato

Steroidal glycoalkaloids (SGAs) existing in most potato tissues are toxic to humans when the fresh weight is over 200 mg kg?1, and high SGAs content would also damage quality of tubers. Sterol side chain reductase 2 (StSSR2) a key enzyme biosynthetic potatoes. To reduce concentration tetraploid cultivated (Solanum tuberosum L.), gene StSSR2 was edited by CRISPR/Cas9 system. A total number 64 mutant lines were generated, mutation efficiency 46%. The percentage insertion 6.25%, deletion 93.75%. Compared wild-type (WT), reduced significantly mutated lowest 54% WT level, which recorded peels line #360. In tuber fleshes, 66% found #378. leaves, 44% level as #204 word, our results showed that could be successfully system SGAs.